• When Mario goes to his room, he is seen playing Kid Icarus on an original Nintendo Entertainment System (NES) console. This is reference is twofold, first Mario playing in his NES is a reference to the opening of the anime film Super Mario Brothers: Great Mission to Rescue Princess Peach (1986) that showed Mario playing a version of Super Mario Bros. (1985) on the original Nintendo Family Computer (Famicom). Second, the game Mario is playing on his NES is Kid Icarus (1986), a Nintendo game that is loosely based on the Greek mythology. In Greek mythology, Icarus was the son of Daedalus, who instructed Icarus to fly neither too low or too high, lest the sea's dampness clog his wings or too high or the sun's heat will melt them. This is a metaphor for the Mario's relationship with his own father who disapproves of Mario's decision to leave his steady job and blames Mario for bringing his brother Luigi along with him. While Mario just wants to make his father proud of him.
• The website shown at the end of the Super Mario Brothers' Plumbing commercial (www.smbplumbing.com) was a real site at the time of the film's release. The website gave the business' phone number as 1-929-556-2746 (1-929-55-MARIO). At the time of the film's release, calling that number would play a recorded message from Mario.
• The film homages also the "Super Smash Bros." video game series, as it can be seen in the fights against Donkey Kong and Bowser and in the training course sequences. Also, Peach is here a strong and skilled fighter like in the ''Super Smash Bros.'' series, and she trains Mario to fight as such to stop Bowser and save Luigi, with many moves taken directly from the popular and beloved fighting spin-off video game series.
• When Luigi arrives in The Dark Lands, he faces a skeleton turtle which he smashes by chance, only for it to reform once again seconds later. This character is Dry Bones, which was introduced for the first time in Super Mario Bros. 3 (1988), appearing only in the various castle levels. Since it's an undead turtle, it can't be defeated, only briefly smashed by the player to allow the time necessary to pass before it come back.
• When Mario meets Peach the first time, she tests Mario's skills at jumping, climbing and running, giving him a mushroom to make him bigger as he was too small for the test. This is the classic Super Mushroom from Super Mario Bros. (1985), used by Mario and Luigi to make them bigger. Super Mushrooms make them capable of destroying bricks in the game level that they normally wouldn't be able to.

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